6MYF
Crystal structure of free human Scribble PDZ1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-05-11 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.976251 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 39.108, 42.824, 91.234 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.617 - 1.600 |
R-factor | 0.1731 |
Rwork | 0.171 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w4f |
RMSD bond length | 0.008 |
RMSD bond angle | 0.994 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.620 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.065 | 0.547 |
Rmeas | 0.077 | 0.683 |
Rpim | 0.030 | 0.339 |
Number of reflections | 10237 | 1290 |
<I/σ(I)> | 18.5 | 1.8 |
Completeness [%] | 97.8 | 87.4 |
Redundancy | 6.2 | 3.7 |
CC(1/2) | 0.999 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M Citric acid pH 3.5, 25% (w/v) PEG 3350 |