6MYD
Structure of zebrafish TRAF6 in complex with STING CTT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-03-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.621, 84.377, 53.720 |
Unit cell angles | 90.00, 115.42, 90.00 |
Refinement procedure
Resolution | 48.520 - 1.399 |
R-factor | 0.1812 |
Rwork | 0.181 |
R-free | 0.19890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lb5 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.859 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.520 | 1.420 |
High resolution limit [Å] | 1.399 | 1.399 |
Rpim | 0.070 | 0.584 |
Number of reflections | 73125 | |
<I/σ(I)> | 4.8 | 1.2 |
Completeness [%] | 99.0 | 94.2 |
Redundancy | 3.5 | 3.2 |
CC(1/2) | 0.989 | 0.495 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES pH 7.5, 1.5 M LiSO4 |