6MYD

Structure of zebrafish TRAF6 in complex with STING CTT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	46.621, 84.377, 53.720
Unit cell angles	90.00, 115.42, 90.00

Refinement procedure

Resolution	48.520 - 1.399
R-factor	0.1812
Rwork	0.181
R-free	0.19890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lb5
RMSD bond length	0.005
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	1.420
High resolution limit [Å]	1.399	1.399
Rpim	0.070	0.584
Number of reflections	73125
<I/σ(I)>	4.8	1.2
Completeness [%]	99.0	94.2
Redundancy	3.5	3.2
CC(1/2)	0.989	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES pH 7.5, 1.5 M LiSO4