6MYA

Crystal structure of InvbP.18715.a.KN11: Influenza hemagglutinin from strain A/Almaty/32/1998

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-05
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	69.650, 244.370, 115.020
Unit cell angles	90.00, 90.09, 90.00

Refinement procedure

Resolution	45.928 - 2.050
R-factor	0.1884
Rwork	0.188
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d8w
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3283)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.928	45.928	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.065	0.027	0.545
Rmeas	0.077	0.032	0.650
Total number of observations	759013
Number of reflections	231107	2544	17159
<I/σ(I)>	12.59	36.46	2.36
Completeness [%]	96.5	92.5	97.2
Redundancy	3.284	3.232	3.277
CC(1/2)	0.998	0.998	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	SEC purified InvbP.18715.a.KN11.PD38330 at 10mg/ml (in 25mM TRIS pH 8, 150mM NaCl) was crystallized by sitting drop vapor diffusion at 14C with an equal volume (0.1:0.1uL) of protein:mother liquor (0.1M MES pH6.5, 15% PEG6000, 5% MPD), then cyoprotected in mother liquor+20% ethylene glycol, before being flash-frozen for data collection. Crystal ID 304576d9 data set jbg0-3