6MXV
The crystal structure of a rhodanese-like family protein from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97818 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.721, 111.108, 50.339 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 48.600 - 1.780 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.18840 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.958 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.061 | 0.490 |
| Rmeas | 0.069 | 0.560 |
| Rpim | 0.031 | 0.266 |
| Number of reflections | 50307 | 2503 |
| <I/σ(I)> | 22.3 | 3.67 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4.4 | 3.8 |
| CC(1/2) | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | PEG3350 25%, 0.2M MgCl2, 0.1M Tris |
