6MXU

Crystal structure of hemagglutinin from influenza virus A/Texas/1/1977 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-19
Detector	NOIR-1
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	197.890, 186.240, 106.960
Unit cell angles	90.00, 110.09, 90.00

Refinement procedure

Resolution	46.373 - 1.850
R-factor	0.1935
Rwork	0.193
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp5
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.274	7.594
Rmeas	0.291	8.116
Rpim	0.097	2.794
Number of reflections	308174	15279
<I/σ(I)>	12
Completeness [%]	99.9	99.9
Redundancy	17.6	16.1
CC(1/2)	0.997	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.056M sodium phosphate monobasic, 1.4M potassium phosphate dibasic