Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.682, 52.589, 125.917 |
| Unit cell angles | 90.00, 123.86, 90.00 |
Refinement procedure
| Resolution | 28.520 - 2.959 |
| R-factor | 0.207099158562 |
| Rwork | 0.205 |
| R-free | 0.24486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 4GBR & 4LDE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.571 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.090 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.167 | 0.525 |
| Number of reflections | 18742 | 1012 |
| <I/σ(I)> | 1.52 | 1.52 |
| Completeness [%] | 92.6 | 75 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.5 | 293.15 | 31-34% PEG400, 100 mM HEPES, pH 7.5, 1% 1,2,3-heptanetriol |
