6MX9
Lysozyme bound to 3-Aminophenol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9202 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.085, 80.085, 37.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.310 - 1.350 |
R-factor | 0.15898 |
Rwork | 0.158 |
R-free | 0.18590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n8z |
RMSD bond length | 0.029 |
RMSD bond angle | 2.533 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.310 | 1.390 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.068 | 0.725 |
Number of reflections | 25655 | |
<I/σ(I)> | 21.9 | 3.2 |
Completeness [%] | 99.7 | 96 |
Redundancy | 12.8 | 11.3 |
CC(1/2) | 0.998 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 10uL of 50mM 3-aminophenol was deposited on a cover slip and allowed to dry. A 20 uL pellet of 4% sodium chloride + 5% ethylene glycol + 10% glycerol in 2% agar was deposited on the cover slip. Adjacent to this was positioned lysozyme (10 ul of 30 mg/ml lysozyme in 100 mM sodium acetate pH 4.6). The cover slip was equilibrated over precipitant containing all the crystallization components. |