6MWN

Crystal structure of hepatitis A virus IRES domain V in complex with Fab HAVx

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033202
Spacegroup name	P 21 2 21
Unit cell lengths	74.149, 100.582, 236.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.607 - 2.838
R-factor	0.1856
Rwork	0.186
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Fab BL3-6 4KZE 3ivk
RMSD bond length	0.009
RMSD bond angle	1.180
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.607	2.940
High resolution limit [Å]	2.838	2.838
Rmerge	0.100
Rmeas	0.109
Rpim	0.042	0.524
Number of reflections	42565	4057
<I/σ(I)>	13.89	1.38
Completeness [%]	99.6	96.4
Redundancy	6.8	6.8
CC(1/2)	0.999	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.2 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 25% PEG 3350