6MWB

NavAb Voltage-gated Sodium Channel, residues 1-239 with mutation T206A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	123.849, 123.849, 190.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.780 - 2.600
R-factor	0.2082
Rwork	0.207
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.006
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	22844
<I/σ(I)>	22
Completeness [%]	99.1
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	1.8 M Ammonium Sulfate 100 mM Sodium Acetate pH 5.8