6MW5

UDP-galactose:glucoside-Skp1 alpha-D-galactosyltransferase with bound UDP and Platinum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.85
Spacegroup name	P 42 21 2
Unit cell lengths	83.780, 83.780, 75.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.686 - 2.100
R-factor	0.1983
Rwork	0.196
R-free	0.24190
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.786
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.13_2998))
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	29424	2114
<I/σ(I)>	28.6
Completeness [%]	97.4	94.8
Redundancy	13.5	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	8-12% PEG4000, 0.4M ammonium sulfate, 0.1M sodium acetate