6MUO
CENP-A nucleosome bound by two copies of CENP-C(CD) and one copy CENP-N(NT)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM CPC |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Software | RELION,PHENIX,SerialEM,Gctf,Coot |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
