6MUJ
Formylglycine generating enzyme bound to copper
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.115834 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 140.008, 140.008, 217.508 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.738 - 2.249 |
R-factor | 0.2031 |
Rwork | 0.202 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q17 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.932 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.738 | 2.330 |
High resolution limit [Å] | 2.249 | 2.249 |
Number of reflections | 11546 | |
<I/σ(I)> | 12.72 | 0.65 |
Completeness [%] | 94.0 | 83.18 |
Redundancy | 10.8 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | Crystals were grown by hanging-drop vapor diffusion at 23C for one week using a 2uL:2uL mixture of protein solution (8 mg/ml in 50 mM Tris 7.5, 0.5 M NaCl, 10% glycerol, 1 mM DTT) and precipitant buffer (100 mM Tris 8.0, 3.2 M ammonium formate, 0.3% octyl-beta-glucoside (w/v), 3.2% 2-butanol (v/v)) in Easy-Xtal trays. Crystals were soaked in 1mM DTT (in precipitant buffer) for 15 minutes, then soaked in CuCl2 (in precipitant buffer) for 30 minutes, and finally, backsoaked and cryoprotected in 20% glycerol (in precipitant buffer). |