6MUE
Voltage-gated sodium channel NaV1.4 IQ domain in complex with Ca2+/Calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 58.580, 24.433, 60.720 |
| Unit cell angles | 90.00, 112.63, 90.00 |
Refinement procedure
| Resolution | 33.070 - 1.900 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4djc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS (October 15, 2015) |
| Data scaling software | XDS (October 15, 2015) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.093 | 0.744 |
| Number of reflections | 14964 | 2235 |
| <I/σ(I)> | 9.66 | 1.68 |
| Completeness [%] | 98.3 | 91.4 |
| Redundancy | 3.81 | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M MES pH 6.0 and 55 % (v/v) isopropanol |
