6MUE
Voltage-gated sodium channel NaV1.4 IQ domain in complex with Ca2+/Calmodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 58.580, 24.433, 60.720 |
Unit cell angles | 90.00, 112.63, 90.00 |
Refinement procedure
Resolution | 33.070 - 1.900 |
R-factor | 0.2159 |
Rwork | 0.214 |
R-free | 0.26030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4djc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | XDS (October 15, 2015) |
Data scaling software | XDS (October 15, 2015) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmeas | 0.093 | 0.744 |
Number of reflections | 14964 | 2235 |
<I/σ(I)> | 9.66 | 1.68 |
Completeness [%] | 98.3 | 91.4 |
Redundancy | 3.81 | 3.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M MES pH 6.0 and 55 % (v/v) isopropanol |