6MTZ
Crystal structure of an uncharacterized protein from Helicobacter pylori G27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-09-14 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.730, 66.740, 200.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.943 - 2.150 |
R-factor | 0.1777 |
Rwork | 0.175 |
R-free | 0.22790 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.753 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.943 | 44.943 | 2.210 |
High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
Rmerge | 0.097 | 0.049 | 0.606 |
Rmeas | 0.101 | 0.051 | 0.647 |
Number of reflections | 45317 | 594 | 3281 |
<I/σ(I)> | 17.92 | 39.48 | 3.49 |
Completeness [%] | 100.0 | 97.2 | 100 |
Redundancy | 13.491 | 11.719 | 8.285 |
CC(1/2) | 0.999 | 0.999 | 0.846 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | Microlytic MCSG1 screen, F3: 200mM Ammonium citrate dibasic pH 5, 20% PEG3350: HepyC.18480.a.B11.PW38477 at 31.62mg/ml. For phasing, a crysta was incubated for 10sec each in reservoir 10% 2.5M NaI in EG and 20% 2.5M NaI in EG (500mM final NaI concentration), and directly vitrified. Tray 301766f3, puck OVZ1-5 |