6MTV
Crystal structure of human Scribble PDZ1:MCC complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 57.403, 55.559, 61.775 |
| Unit cell angles | 90.00, 116.12, 90.00 |
Refinement procedure
| Resolution | 39.253 - 2.597 |
| R-factor | 0.2494 |
| Rwork | 0.248 |
| R-free | 0.26570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vwi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.526 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.253 | 2.710 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.072 | 0.976 |
| Rpim | 0.072 | 0.975 |
| Number of reflections | 5477 | 617 |
| <I/σ(I)> | 8.1 | 0.553 |
| Completeness [%] | 97.2 | 95 |
| Redundancy | 2.8 | 2.7 |
| CC(1/2) | 0.997 | 0.553 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293.15 | polyethylene glycol 1000, lithium sulfate monohydrate, phosphate/citrate |
