6MTK
Crystal structure of Tryptophanyl-tRNA synthetase from Elizabethkingia anophelis NUHP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-09-14 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.070, 105.920, 94.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.095 - 2.000 |
R-factor | 0.204 |
Rwork | 0.200 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3TZL as per MoRDa |
RMSD bond length | 0.006 |
RMSD bond angle | 0.768 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.095 | 47.095 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.041 | 0.026 | 0.507 |
Rmeas | 0.043 | 0.027 | 0.540 |
Number of reflections | 22221 | 288 | 1643 |
<I/σ(I)> | 33.22 | 86.96 | 4.19 |
Completeness [%] | 99.3 | 99 | 99.9 |
Redundancy | 12.517 | 11.854 | 8.363 |
CC(1/2) | 0.999 | 0.999 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 22.6 mg/mL ElanA.00743.a.B1.PS38400 + Molecular Dimensions Morpheus screen, condition C10 (10% w/v PEG8000, 20% v/v ethylene glycol, 30 mM each NPS, 100 mM bicine/Trizma base, pH 8.5, sodium nitrate, 0.3 M disodium hydrogen phosphate, 0.3 M ammonium sulfate), cryoprotectant: direct, Tray 299155c10, puck OVZ1-7 |