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6MTK

Crystal structure of Tryptophanyl-tRNA synthetase from Elizabethkingia anophelis NUHP1

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU FR-E+ SUPERBRIGHT
Temperature [K]100
Detector technologyCCD
Collection date2018-09-14
DetectorRIGAKU SATURN 944+
Wavelength(s)1.5418
Spacegroup nameC 2 2 21
Unit cell lengths65.070, 105.920, 94.190
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution47.095 - 2.000
R-factor0.204
Rwork0.200
R-free0.24510
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3TZL as per MoRDa
RMSD bond length0.006
RMSD bond angle0.768
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMoRDa
Refinement softwarePHENIX
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]47.09547.0952.050
High resolution limit [Å]2.0008.9402.000
Rmerge0.0410.0260.507
Rmeas0.0430.0270.540
Number of reflections222212881643
<I/σ(I)>33.2286.964.19
Completeness [%]99.39999.9
Redundancy12.51711.8548.363
CC(1/2)0.9990.9990.950
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.529022.6 mg/mL ElanA.00743.a.B1.PS38400 + Molecular Dimensions Morpheus screen, condition C10 (10% w/v PEG8000, 20% v/v ethylene glycol, 30 mM each NPS, 100 mM bicine/Trizma base, pH 8.5, sodium nitrate, 0.3 M disodium hydrogen phosphate, 0.3 M ammonium sulfate), cryoprotectant: direct, Tray 299155c10, puck OVZ1-7

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PDB entries from 2024-11-13

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