6MTF

D7 protein from Phlebotomus duboscqi, native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97910
Spacegroup name	P 21 21 2
Unit cell lengths	143.890, 101.560, 33.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.487 - 1.920
R-factor	0.1659
Rwork	0.164
R-free	0.20840
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.487	1.960
High resolution limit [Å]	1.920	1.920
Rmerge	0.078	0.390
Number of reflections	38751	2811
<I/σ(I)>	28.2	6.33
Completeness [%]	99.9	98.7
Redundancy	11.85
CC(1/2)	0.990	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	20% PEG 6000, 0.1 M MES