6MT5
Crystal Structure of HLA-B*37:01 in complex with NP338-V6L influenza peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.954 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.861, 82.040, 110.202 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.373 - 1.550 |
R-factor | 0.1839 |
Rwork | 0.183 |
R-free | 0.20670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gso |
RMSD bond length | 0.005 |
RMSD bond angle | 0.967 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.040 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 67762 | 9785 |
<I/σ(I)> | 9.7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG6000, 0.2M NaCl, 0.1M Na citrate pH 6.5 |