6MT4
Crystal Structure of HLA-B*37:01 in complex with NP338-L7S influenza peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.973, 81.720, 110.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.280 - 1.550 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gso |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.1) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.720 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.166 | 0.680 |
Total number of observations | 457612 | 24177 |
Number of reflections | 66469 | 3333 |
<I/σ(I)> | 7.9 | 2.1 |
Completeness [%] | 98.1 | 100 |
Redundancy | 6.9 | 7.3 |
CC(1/2) | 0.953 | 0.808 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG6000, 0.2M NaCl, 0.1M Na citrate pH 6.5 |