6MR0
Crystal Structure of the All-trans Retinal Bound R111K:Y134F:T54V:R132Q:P39Q:R59Y:L121E Human Cellular Retinoic Acid Binding Protein II Mutant After 5 Minutes UV irradiation at 2.6 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.476, 58.476, 99.552 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.499 - 2.649 |
R-factor | 0.1996 |
Rwork | 0.191 |
R-free | 0.27360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.005 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.499 | 2.744 |
High resolution limit [Å] | 2.649 | 2.650 |
Rmerge | 0.150 | 0.800 |
Rpim | 0.324 | |
Number of reflections | 6063 | 569 |
<I/σ(I)> | 11.25 | 2 |
Completeness [%] | 99.6 | 97.43 |
Redundancy | 10.7 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, BTP, Sodium Fluoride |