6MQY
Crystal Structure of the 13-cis Product of All-trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121E Mutant of Human Cellular Retinoic Acid Binding Protein II Irradiated with 400 nm Laser (30 seconds) at 1.9 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.343, 58.343, 99.865 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.171 - 1.900 |
R-factor | 0.1911 |
Rwork | 0.189 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.008 |
RMSD bond angle | 0.871 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.171 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.047 | 0.359 |
Rmeas | 0.481 | |
Number of reflections | 16010 | 1552 |
<I/σ(I)> | 35.49 | 3.3 |
Completeness [%] | 99.2 | 98.66 |
Redundancy | 6.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 3350, D-Malic acid |