6MQX
Crystal Structure of All-trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121E Human Cellular Retinoic Acid Binding Protein II Irradiated with 400 nm Laser (30 seconds) and Subsequently Dark Adapted (25 minutes) at 2.0 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2016-09-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.501, 58.501, 99.982 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.584 - 2.010 |
R-factor | 0.2022 |
Rwork | 0.199 |
R-free | 0.23330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.007 |
RMSD bond angle | 0.863 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.584 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.065 | 0.620 |
Rmeas | 0.079 | 0.708 |
Number of reflections | 13679 | 1343 |
<I/σ(I)> | 44.63 | 3.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 9.1 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, DL-malic acid |