6MQW

Crystal Structure of All-trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121E Human Cellular Retinoic Acid Binding Protein II Irradiated with 400 nm laser (30 seconds) and subsequently dark adapted (10 minutes) at 2.1 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97911
Spacegroup name	P 31 2 1
Unit cell lengths	58.586, 58.586, 100.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.645 - 2.090
R-factor	0.1865
Rwork	0.183
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yfp
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.645	2.120
High resolution limit [Å]	2.090	2.090
Rmerge	0.067	0.677
Rmeas	0.093	0.754
Number of reflections	12273	1205
<I/σ(I)>	36.75	3.8
Completeness [%]	99.9	100
Redundancy	9.5	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 3350, DL-malic acid