6MQP

Structure of HIV-1 CA T210K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979200
Spacegroup name	P 6
Unit cell lengths	91.767, 91.767, 57.216
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.434 - 3.296
R-factor	0.2067
Rwork	0.205
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xfx
RMSD bond length	0.010
RMSD bond angle	1.156
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.490
High resolution limit [Å]	3.296	3.296
Number of reflections	4264
<I/σ(I)>	7.55	1.67
Completeness [%]	99.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.8	293	15% PEG3350, 0.1 M sodium iodide, 0.1 M sodium cacodylate