6MQM
Vaccine-elicited NHP FP-targeting neutralizing antibody DF1W-a.01 in complex with HIV fusion peptide (residue 512-519)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.906, 147.933, 102.017 |
| Unit cell angles | 90.00, 93.02, 90.00 |
Refinement procedure
| Resolution | 43.517 - 3.484 |
| R-factor | 0.2025 |
| Rwork | 0.200 |
| R-free | 0.24640 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
| High resolution limit [Å] | 3.484 | 9.480 | 3.500 |
| Rmerge | 0.206 | 0.072 | 0.561 |
| Rmeas | 0.246 | 0.086 | 0.669 |
| Rpim | 0.133 | 0.046 | 0.359 |
| Number of reflections | 27904 | 1455 | 1377 |
| <I/σ(I)> | 3.5 | ||
| Completeness [%] | 96.8 | 97.6 | 98 |
| Redundancy | 3.3 | 3.3 | 3.3 |
| CC(1/2) | 0.989 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M KSCN and 20% w/v PEG 3350 |
