6MQK

Crystal Structure of GTPase Domain of Human Septin 12 in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 1
Unit cell lengths	46.960, 69.250, 88.230
Unit cell angles	75.45, 89.46, 76.43

Refinement procedure

Resolution	45.586 - 2.190
R-factor	0.1865
Rwork	0.184
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mq9
RMSD bond length	0.005
RMSD bond angle	0.798
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.590	2.250
High resolution limit [Å]	2.190	2.190
Rmerge	0.108
Rpim	0.068	0.607
Number of reflections	52030	3910
<I/σ(I)>	6.9	1.3
Completeness [%]	96.7	96.3
Redundancy	3.4	3.5
CC(1/2)	0.995	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.1 M bicine/Trizma base pH 8.5, 30mM magnesium chloride, 30mM calcium chloride