6MQJ
Crystal Structure of the All-Trans Retinal bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121Q mutant of Human Cellular Retinoic Acid Binding Protein II Irradiated with 532 nm Laser for 10 minutes at 2.1 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-04 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 59.238, 59.238, 99.393 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.587 - 2.125 |
R-factor | 0.1942 |
Rwork | 0.189 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.007 |
RMSD bond angle | 0.933 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.587 | 2.201 |
High resolution limit [Å] | 2.125 | 2.125 |
Rmerge | 0.060 | 0.515 |
Rmeas | 0.685 | |
Number of reflections | 11843 | 1167 |
<I/σ(I)> | 19.3 | |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 8.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG3350, DL-malic acid |