6MQ7

Crystal structure of CLASP1 TOG2 domain at 1.78A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-19
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	51.606, 67.352, 81.022
Unit cell angles	90.00, 98.25, 90.00

Refinement procedure

Resolution	38.090 - 1.780
R-factor	0.1789
Rwork	0.177
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k92
RMSD bond length	0.008
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.090	1.830
High resolution limit [Å]	1.780	1.780
Rmeas	0.095	0.278
Number of reflections	51928	4492
<I/σ(I)>	15.1
Completeness [%]	98.1	85.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	300	equal volume of 10 mg/ml hCLASP1 TOG2 and well solution containing 22% PEG3350, 200mM Sodium citrate, pH 8.25 were mixed together and equilibrated over 1 ml of well solution., VAPOR DIFFUSION, HANGING DROP, temperature 300K