6MQ5
Structure of human CLASP1 TOG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-08-06 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97964 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.975, 114.022, 46.649 |
| Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
| Resolution | 32.947 - 2.146 |
| R-factor | 0.1949 |
| Rwork | 0.190 |
| R-free | 0.24370 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.146 | 2.150 |
| Rmeas | 0.155 | 0.646 |
| Rpim | 0.080 | 0.348 |
| Number of reflections | 41883 | 3667 |
| <I/σ(I)> | 9.6 | 1.96 |
| Completeness [%] | 92.3 | 81.2 |
| Redundancy | 3.5 | 3 |
| CC(1/2) | 1.000 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | equal volume of 10 mg/ml hCLASP1 TOG1 and well solution containing 30% PEG600, 100mM MES, pH 6.5, 10% glycerol were mixed together and equilibrated over 1 ml of well solution, crystals that were originally crystallized in 1.5 M sodium malonate, pH 6.25, 18C, were used for microseeding |
