6MPZ

Crystal structure of a double glycine motif protease from AMS/PCAT transporter in complex with the leader peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	80
Detector technology	CCD
Collection date	2018-02-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	37.888, 119.426, 76.522
Unit cell angles	90.00, 93.84, 90.00

Refinement procedure

Resolution	50.010 - 2.000
R-factor	0.23606
Rwork	0.234
R-free	0.26829
RMSD bond length	0.015
RMSD bond angle	1.815
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.400	1.985
High resolution limit [Å]	1.980	1.980
Number of reflections	47187
<I/σ(I)>	9.3
Completeness [%]	99.8
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	282	0.02 M D-glucose, 0.02 M D-mannose, 0.02 M D-galactose, 0.02 M L-fucose, 0.02 M D-xylose, 0.0 2M N-acetyl-D-glucosamine, 0.05 M Tris and BICINE pH 8.5, 20% v/v polyethylene glycol 500 monomethyl ether, 10% w/v polyethylene glycol 20000, and 8% v/v formamide