6MPK
Crystal Structure of the 13-cis Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121E:A32Y Mutant of Human Cellular Retinoic Acid Binding Protein II in the Dark at 1.58 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.761, 58.761, 99.174 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.515 - 1.580 |
| R-factor | 0.2039 |
| Rwork | 0.203 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.515 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.065 | 0.605 |
| Number of reflections | 27796 | 2743 |
| <I/σ(I)> | 36 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, D-Malic acid |
