6MOW
Crystal Structure of the apo R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121Q mutant of Human Cellular Retinoic Acid Binding Protein II at 2.3 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-04 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 57.561, 57.561, 102.042 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.656 - 2.354 |
R-factor | 0.1999 |
Rwork | 0.193 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.007 |
RMSD bond angle | 0.951 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.660 | 2.439 |
High resolution limit [Å] | 2.354 | 2.354 |
Rmerge | 0.069 | 0.580 |
Rmeas | 0.101 | 0.778 |
Number of reflections | 8421 | 742 |
<I/σ(I)> | 32.3 | |
Completeness [%] | 98.3 | 88.12 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG3350, Sodium Malonate |