6MOU
Bacteroides intestinalis feruloyl esterase, Bacint_01033
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-07 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 95.206, 95.206, 202.609 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.369 - 2.240 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.23600 |
Structure solution method | SAD |
Data reduction software | d*TREK |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.370 | 2.320 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.180 | 0.780 |
Number of reflections | 98838 | |
<I/σ(I)> | 7.5 | |
Completeness [%] | 100.0 | |
Redundancy | 5.8 | |
CC(1/2) | 0.987 | 0.573 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Bis-Tris pH 6.5, 20% PEG 1,500. Hampton PegRx screen well B8 |