6MOS
Structure of thioredoxin 1 from the thermophilic eubacterium Thermosipho africanus TCF52B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-04 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.454, 41.023, 55.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.057 - 1.800 |
R-factor | 0.212317242433 |
Rwork | 0.211 |
R-free | 0.24563 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.843 |
Data scaling software | SCALA |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.020 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 8989 | 1198 |
<I/σ(I)> | 28.2 | 7.3 |
Completeness [%] | 99.1 | 95 |
Redundancy | 6.1 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 289 | 0.2 M MgCl2 , 0.1 M bis-tris pH 6.5, 25%(w/v) PEG 3350 |