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6MOQ

Crystal Structure of the All-trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121W Mutant of Human Cellular Retinoic Acid Binding Protein II in the Dark at 2.2 Angstrom Resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]100
Detector technologyCCD
Collection date2016-10-18
DetectorRAYONIX MX300HE
Wavelength(s)0.97872
Spacegroup nameP 31 2 1
Unit cell lengths58.117, 58.117, 99.629
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution35.405 - 2.198
R-factor0.2342
Rwork0.229
R-free0.27700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4yfp
RMSD bond length0.008
RMSD bond angle1.034
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX ((1.14_3260))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.280
High resolution limit [Å]2.1982.240
Rmerge0.0770.578
Rpim0.0560.417
Number of reflections103251017
<I/σ(I)>22.81.6
Completeness [%]99.398.25
Redundancy4.34.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP277PEG 3350, sodium Malonate

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