6MNS
Rhesus macaque anti-HIV V3 antibody DH753 with gp120 V3 ZAM18 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2017-06-06 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.920, 138.315, 71.678 |
Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
Resolution | 38.772 - 2.700 |
R-factor | 0.1769 |
Rwork | 0.170 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Previously solved DH753 structure (also in submission) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.055 | 0.278 |
Number of reflections | 25304 | 1299 |
<I/σ(I)> | 17.6 | 3 |
Completeness [%] | 99.5 | 100 |
Redundancy | 4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium dihydrogen phosphate, 20% PEG 3,350 |