6MNI
Structure of the tandem CACHE domain of PscC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2017-08-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.953724086285 |
Spacegroup name | P 1 |
Unit cell lengths | 34.244, 53.916, 73.312 |
Unit cell angles | 104.79, 91.20, 92.71 |
Refinement procedure
Resolution | 48.272 - 1.696 |
R-factor | 0.1641 |
Rwork | 0.162 |
R-free | 0.19990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c8c |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | BALBES |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.272 | 48.270 | 1.730 |
High resolution limit [Å] | 1.696 | 8.980 | 1.700 |
Rmerge | 0.075 | 0.054 | 0.463 |
Rmeas | 0.089 | 0.063 | 0.547 |
Rpim | 0.047 | 0.033 | 0.288 |
Number of reflections | 54175 | 362 | 2662 |
<I/σ(I)> | 7.2 | 1.4 | |
Completeness [%] | 96.6 | 97.3 | 89.2 |
Redundancy | 3.6 | 3.6 | 3.4 |
CC(1/2) | 0.995 | 0.994 | 0.816 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Bis-tris pH 5.5 and 30% PEG3350 |