6MNA
Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-08-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | I 4 |
Unit cell lengths | 79.554, 79.554, 59.959 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.250 - 1.750 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.21750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e5f |
RMSD bond length | 0.012 |
RMSD bond angle | 1.242 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.250 | 1.830 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 3.960 | |
Rmeas | 4.000 | |
Rpim | 0.962 | |
Number of reflections | 19157 | 2756 |
<I/σ(I)> | 11.1 | 1.5 |
Completeness [%] | 99.3 | 98.5 |
Redundancy | 17.6 | 17.8 |
CC(1/2) | 0.999 | 0.643 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M sodium citrate, pH 6.5, 1.9 M ammonium sulfate |