6MN6

Crystal structure of the cytosolic domain of human CNNM3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.6235
Spacegroup name	P 32 2 1
Unit cell lengths	128.063, 128.063, 111.316
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.750 - 3.360
Rwork	0.217
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k23 6dfd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12-2829)
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	12603	90
<I/σ(I)>	12.2	0.5
Completeness [%]	100.0	100
Redundancy	11.9	9.7
CC(1/2)		0.203

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris, pH 8.5; 0.5 M succinic acid, pH 7.0