6MMP

Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 0.1 millimolar EDTA, and at pH 8.0

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K2 BASE (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	6.88
Software	PHENIX,Coot,RELION,SerialEM,Gctf,UCSF Chimera,PyMOL
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION