6MMG
Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 1 millimolar EDTA, and at pH 7.4
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 BASE (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 6.23 |
Software | PHENIX,Coot,RELION,cisTEM,SerialEM,Gctf,UCSF Chimera,PyMOL |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |