6MMC

Carbon regulatory PII-like protein SbtB from Cyanobium sp. 7001 bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 63 2 2
Unit cell lengths	68.632, 68.632, 89.052
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.440 - 1.420
Rwork	0.172
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mm2
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.440	1.440
High resolution limit [Å]	1.420	1.420
Rmerge	0.105
Rmeas	0.011
Rpim	0.024
Number of reflections	24053	1180
<I/σ(I)>	22.6
Completeness [%]	100.0	100
Redundancy	37.8	36.3
CC(1/2)	1.000	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.5 M Ammonium Sulfate