6MM2
Carbon regulatory PII-like protein SbtB from Cyanobium sp. 7001 bound to ATP and calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | H 3 |
| Unit cell lengths | 52.058, 52.058, 95.214 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.040 - 1.040 |
| Rwork | 0.145 |
| R-free | 0.16320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o3p |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.045 | 1.060 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.039 | 1.337 |
| Rmeas | 0.043 | |
| Rpim | 0.020 | |
| Number of reflections | 44144 | 2573 |
| <I/σ(I)> | 0.999 | |
| Completeness [%] | 95.3 | 50.9 |
| Redundancy | 9 | 2.2 |
| CC(1/2) | 0.999 | 0.405 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30 % 2-methyl-2,4-pentanediol 0.02 M calcium chloride 0.1 M sodium acetate-acetic acid pH 4.6 |
