6MM2
Carbon regulatory PII-like protein SbtB from Cyanobium sp. 7001 bound to ATP and calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | H 3 |
Unit cell lengths | 52.058, 52.058, 95.214 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.040 - 1.040 |
Rwork | 0.145 |
R-free | 0.16320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o3p |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.045 | 1.060 |
High resolution limit [Å] | 1.040 | 1.040 |
Rmerge | 0.039 | 1.337 |
Rmeas | 0.043 | |
Rpim | 0.020 | |
Number of reflections | 44144 | 2573 |
<I/σ(I)> | 0.999 | |
Completeness [%] | 95.3 | 50.9 |
Redundancy | 9 | 2.2 |
CC(1/2) | 0.999 | 0.405 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30 % 2-methyl-2,4-pentanediol 0.02 M calcium chloride 0.1 M sodium acetate-acetic acid pH 4.6 |