6MM1

Structure of the cysteine-rich region from human EHMT2

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Detector technology	PIXEL
Collection date	2017-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28
Spacegroup name	P 1
Unit cell lengths	47.996, 54.358, 59.317
Unit cell angles	91.68, 100.92, 96.89

Refinement procedure

Resolution	24.510 - 1.900
R-factor	0.176
Rwork	0.174
R-free	0.22300
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.746
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.507	1.930
High resolution limit [Å]	1.897	1.900
Number of reflections	43917	4011
<I/σ(I)>	7.6	1.6
Completeness [%]	95.4	89.9
Redundancy	2.2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M potassium sodium tartrate 18% PEG 3350 0.1M HEPES, pH 8.0
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M potassium sodium tartrate 18% PEG 3350 0.1M HEPES, pH 8.0
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M potassium sodium tartrate 18% PEG 3350 0.1M HEPES, pH 8.0