6MLX

Crystal structure of T. pallidum Leucine Rich Repeat protein (TpLRR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9202
Spacegroup name	P 43 21 2
Unit cell lengths	104.079, 104.079, 185.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.308 - 2.000
R-factor	0.2366
Rwork	0.234
R-free	0.28150
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.927
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.900	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	67107
<I/σ(I)>	15.1
Completeness [%]	96.2
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Bis-Tris HCl, pH 6.5 and 25% PEG 3350