6MLT
Crystal structure of the V. cholerae biofilm matrix protein Bap1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.25414 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.951, 70.951, 303.957 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.640 - 1.900 |
R-factor | 0.1617 |
Rwork | 0.161 |
R-free | 0.17620 |
Structure solution method | SIRAS |
RMSD bond length | 0.004 |
RMSD bond angle | 0.641 |
Data reduction software | XDS (20170615) |
Data scaling software | Aimless (0.5.32) |
Phasing software | AutoSol (1.11.1) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.640 | 47.640 | 1.940 |
High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
Rmerge | 0.114 | 0.070 | 0.639 |
Rmeas | 0.120 | 0.073 | 0.738 |
Rpim | 0.034 | 0.021 | 0.356 |
Number of reflections | 62568 | 710 | 3772 |
<I/σ(I)> | 13.8 | ||
Completeness [%] | 99.7 | 99.2 | 96.2 |
Redundancy | 11.1 | 11.2 | 3.8 |
CC(1/2) | 0.998 | 0.997 | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M sodium citrate, 10% PEG 3350 |