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6MLN

Crystal structure of the periplasmic Lysine-, Arginine-, Ornithine-binding protein (LAO) S72A mutant from Salmonella typhimurium complexed with arginine

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007 HF
Temperature [K]100
Detector technologyPIXEL
Collection date2018-04-16
DetectorDECTRIS PILATUS 200K
Wavelength(s)1.5418
Spacegroup nameP 21 21 21
Unit cell lengths36.804, 59.050, 115.370
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution32.225 - 1.721
R-factor0.1462
Rwork0.144
R-free0.17470
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1laf
RMSD bond length0.006
RMSD bond angle0.999
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwarePHENIX (1.9_1692)
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.750
High resolution limit [Å]1.7201.720
Rmerge0.0650.385
Number of reflections541162679
<I/σ(I)>44.16
Completeness [%]100.099.8
Redundancy9.55.7
CC(1/2)0.910
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.5291.150.2 M Sodium acetate trihydrate. 0.1 M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8,000

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