6ML5
ZBTB24 Zinc Fingers 4-8 with 19+1mer DNA Oligonucleotide (Sequence 4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-25 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.009, 68.237, 48.575 |
| Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
| Resolution | 27.911 - 1.650 |
| R-factor | 0.2267 |
| Rwork | 0.226 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ml4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.465 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.911 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.056 | |
| Rpim | 0.028 | 0.546 |
| Number of reflections | 35444 | 3143 |
| <I/σ(I)> | 14.5 | 1.2 |
| Completeness [%] | 98.0 | 86.7 |
| Redundancy | 4.5 | |
| CC(1/2) | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 25% PEG3350, 0.1 M HEPES, pH 7.5, 0.2 M ammonium acetate |
