6ML3
ZBTB24 Zinc Fingers 4-8 with 19+1mer DNA Oligonucleotide (Sequence 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.297, 68.757, 50.010 |
Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
Resolution | 38.361 - 1.683 |
R-factor | 0.2263 |
Rwork | 0.225 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ml2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.525 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.361 | 1.740 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.099 | 0.358 |
Rpim | 0.039 | 0.188 |
Number of reflections | 48340 | 634 |
<I/σ(I)> | 14.6 | 2.6 |
Completeness [%] | 73.9 | 18 |
Redundancy | 5.7 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 292 | 20% PEG3350, 0.2 M sodium malonate, pH 5.0 |