6MKX

Crystal structure of the periplasmic Lysine-, Arginine-, Ornithine-binding protein (LAO) R77A mutant from Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-12
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	35.001, 76.750, 86.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.081 - 2.284
R-factor	0.1962
Rwork	0.191
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lao
RMSD bond length	0.010
RMSD bond angle	1.061
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.284	2.284
Rmerge	0.109	0.294
Number of reflections	11886	1009
<I/σ(I)>	24.8	3.9
Completeness [%]	99.6	91.4
Redundancy	7.2	2.4
CC(1/2)		0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.2 M Sodium acetate trihydrate 0.1 M Sodium cacodylate trihydrate 30% w/v Polyethylene glycol 8,000